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. 2022 Jul 12;8:317. doi: 10.1038/s41420-022-01105-2

Fig. 4. Trp53 is indispensable for chondrocyte proliferation, matrix production, and maturation.

Fig. 4

A The proliferation of primary chondrocytes from Col2-Cre;Trp53f/f mice and controls was identified at different times as shown by WST-1 assay. B, C Colony formation (B) and quantitative analysis of the colony numbers as shown (C). D, E BrdU staining (D) and quantitative analysis of BrdU+ cells (E) in the tibiae from Col2-Cre;Trp53f/f mice and control at P0. Scale bars, 25 μm. F MMP13 staining at P0 tibiae as indicated. Scale bars, 75 μm. G Representative fluorescence images of Col10a1 at P0 tibiae as indicated. Scale bars, 75 μm. H The mRNA level of chondrocyte differentiation markers in primary chondrocytes as indicated. I, J The primary chondrocytes were cultured by micromass in chondrogenic medium as indicated. After 7 days’ culture, the macromass were stained by the alcian blue solution as shown. The relative fold change of intensity was identified (J). *P < 0.05, **P < 0.01, ***P < 0.001.