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. 2022 Jul 13;8:81. doi: 10.1038/s41378-022-00401-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a A 50:50 beam splitter delivered two image planes with two different focal points. A transmitted white light source with a green bandpass filter delivered illumination with minimized chromatic aberration. b Schematic and experimental result of the dual-camera imaging planes: (i) Camera #1 focused at the center height of microspheres, as shown by the pink focal point, (ii) Camera #2 focused on the virtual image plane, as shown by the yellow focal point. Each scale bar is 5 µm. c Schematic figure of the acoustofluidic nanoscope device. A circular-shape piezoelectric transducer is bonded onto a glass cover slip. Induced acoustic energy pushed microspheres to scan the sample surface.