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. 2022 Jul 13;13:4049. doi: 10.1038/s41467-022-31543-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — An overview of the nine major steps of SURRO-seq is schematically presented. MM, mismatches; ON, on-target; OT, off-targets; PBS, primer binding sites; BB, BsmBI binding site; Sp, spacer; Scr, SpCas9 gRNA scaffold; BC, barcodes; PS, protospacer; PAM, protospacer adjacent motif; 4r, 4 bp downstream sequences; GGA, Golden Gate Assembly; MOI, multiplexity of infection; WT, wildtype; Results presented in Step 9 are indel frequencies captured by SURRO-seq for RGN VEGFA T3.