Fig. 3. Single-cell chromatin conformations are well captured by the model 3D structures, especially by phase-separation based ones.

a Microscopy single-cell chromatin structures of the IMR90 locus⁶ (left) are associated to their best matching single-molecule conformation in each model via the minimum RMSD criterion. Here two examples of best match are shown for each model type. b Less than 5% of the best-matching experiment-model pairs have an RMSD above the 1st decile of the control distribution. c The variability of microscopy single-molecule structures is measured by the distribution of r' correlations between pairs of distance matrices and is compared to the variability of in-silico structures. The r' distribution of the SBS model is statistically indistinguishable from the experimental one (two-sided Mann–Whitney test p-value = 0.362). The boxplots represent the median, interquartile ranges, whiskers within 1.5 times the interquartile range. n = 1000 independent single-molecule conformations for each model. Source data are provided as a Source Data file.