Fig. 6.

Akt signaling participates in aberrant force-mediated OA pathogenesis. a Akt signaling was activated in Ad-NR4A1-infected chondrocytes. b Treatment with Akt pathway inhibitor LY294002 effectively inhibited chondrocyte apoptosis induced by NR4A1. c Akt pathway inhibition partially rescued NR4A1-induced chondrocyte injury. d Representative markers for OA evaluation were investigated by western blot assay. e Aberrant mechanical force induced Akt signaling pathway activation. f Pretreatment with LY294002 prevented SNP-induced apoptosis activities in primary chondrocytes. g Akt pathway inhibition by LY294002 partially attenuated FSS-induced chondrocyte injury. h Representative markers for evaluating cartilage pathogenesis were detected. *P < 0.05; **P < 0.01; ***P < 0.001