Skip to main content
. 2022 Jul 13;13:4046. doi: 10.1038/s41467-022-31519-6

Fig. 1. Cytotoxic CD4+ T-cell frequency in synovial fluid of ACPA− and ACPA+ RA patients.

Fig. 1

a Representative flow cytometry dot plot staining of effector molecules, receptors and transcription factors associated with cytotoxic functions in CD4+ T cells from synovial fluid (SF) from ACPA− (upper panel) and ACPA+ (lower panel) RA patients, quantified in b, (ACPA−, n = 7–9) (ACPA+ , n = 10–12). Line represents median, two-tailed Mann–Whitney U test, P = 0.0072 (%GZMB+PRF1+), ns (%GZMA), P = 0.0018 (%Hobit), ns (%Eomes), P = 0.0036 (%NKG7-high), P = 0.0007 (%GPR56), ns: not significant. c Correlation between the frequency of GZMB+ PRF1+ in CD4+ T cells in SF and the level of serum anti-CCP (cyclic citrullinated peptide) antibodies, n = 21, Spearman two-tailed test, P = 0.0070. d Correlation between the frequency of GPR56 in CD4+ T cells in SF and the level of anti-CCP (cyclic citrullinated peptide) antibodies, n = 21, Spearman two-tailed test, P < 0.0001. a–d Data are from a pool of nine independent experiments where a circle is a single replicate. Blue dots indicate ACPA− RA SF and red dots indicate ACPA+ RA SF.