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. 2022 Jul 13;12:11962. doi: 10.1038/s41598-022-16258-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of Ki-67 and EdU during liver regeneration. (a) Immunofluorescence for Ki-67 (red), HMGB2 (green) and HNF-4α (blue) in WT and HMGB2-KO mouse livers after PHx. Arrows indicate HMGB2- and Ki-67-positive hepatocytes. (b) EdU (green) and DAPI (blue) staining in WT and HMGB2-KO mouse livers after PHx. Scale bar 20 μm. Counting results of Ki-67-positive cells (c) and EdU-positive cells (d) in WT and HMGB2-KO mouse livers. Data represent the mean ± SEM from 5–6 mice per group. Asterisks indicate statistically significant differences (*p < 0.05).