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. 2022 Jul 13;8:318. doi: 10.1038/s41420-022-01089-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Representative confocal images of DG of control, 10’ hypoxia, 20’ hypoxia, 30’ hypoxia, 60’ hypoxia, permanent ischemia groups. Single staining of Nestin (A, B, C, D, E, F); NeuN (G, H, I, J, K, L), Hoechst (M, N, O, P, Q, R), and merge (S, T, U, V, W, X) in mouse dentate gyrus of six groups. Scale bar 200 μm. Rectangular box indicates area of high magnification ×400 zoom. Arrows indicate radial glial-like Nestin expressing cells. B Quantification of Nestin fluorescence intensity in dentate gyrus (expressed in arbitrary units, AU). Data are expressed as mean ± SD (n = 4–5). *P < 0.05 versus control mice. P-values were obtained by using one-way ANOVA with Newman–Keuls correction for multiple comparisons.