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. 2022 Jul 13;12:11933. doi: 10.1038/s41598-022-16106-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Localization of Pcdh9 proteins in cultured hippocampal neurons. (a–c) In 5-days in vitro (5-DIV) cultures, Pcdh9 protein was often localized at contact sites between neurites (arrows). (d–o) In 20-DIV cultures, Pcdh9 protein was often localized at synapses (arrowheads) in addition to other regions of neurites. Synaptic localization was not changed in normal medium (d, g, j, m), medium containing bicuculline (e, h, k, n), and TTX (f, i, l, o). Scale bars, 10 μm in (a–c), 10 μm in (d–f) and 10 μm in (g–o).