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. 2022 Jul 13;13:4061. doi: 10.1038/s41467-022-31574-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative images of immunofluorescence analysis show spindle assembly in mitotic cells by immunostaining for acetylated tubulin (Ac-tubulin, red) and pericentrin (green). Chromosomes are visualized with DAPI (blue). White scale bar: 5 μm. b–d Bar graphs display the percentage of dividing cells displaying normal, disrupted or multipolar spindle mitosis under control (Ctrl = si-negative-POOL or sh-scr) condition and DYNC1I2- or HHIP-AS1-knockdown using siRNAs for transient knockdown in Daoy (b) or two independent shRNAs (sh-HHIP-AS1#1 and sh-HHIP-AS1#2) for stable HHIP-AS1 knockdown in Daoy (c) and in CHLA-266 (d). e Bar graphs showing the percentage of dividing cells displaying normal, disrupted or multipolar spindle mitosis under control (Ctrl) condition or upon rescue of DYNC1I2 expression by endogenous gene transcriptional activation through CRISPR-Cas9 technology in the context of transient HHIP-AS1 knockdown (DYNC1I2 OE + si-HHIP-AS1) in Daoy cells. f Bar graphs showing the percentage of dividing cells displaying normal, disrupted or multipolar spindle mitosis under control (Ctrl) condition or upon rescue of HHIP-AS1 expression by endogenous gene transcriptional activation through CRISPR-Cas9 technology in the context of transient HHIP-AS1 knockdown (HHIP-AS1 OE + si-HHIP-AS1) in Daoy cells. All bar graph values are representative of n at least ten independent experiments (with a total n > 50 counted mitotic cells) and data are shown as mean ± SEM. Student’s two-sided t-test; ***p < 0.001; **p < 0.01; *p < 0.05; n.s. not significant. Source data and exact p-values are provided as a “Source Data file”.