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Isoelectric focusing of the crude culture filtrate from S. rolfsii and the purified CDH preparation. (A) Lane 1 shows purified CDH (activity stained as for panel B). On the silver-stained gel, lane 2 represents purified CDH, lane 3 represents pI standards (Amersham-Pharmacia), and lane 4 represents the culture supernatant. (B) Gel incubated with DCIP and lactose (activity staining). Lane 5 shows purified CDH and lane 6 shows the supernatant.
