. 2001 Apr;67(4):1766–1774. doi: 10.1128/AEM.67.4.1766-1774.2001

TABLE 1.

Purification of cellobiose dehydrogenase from S. rolfsii

Purification stage	Sp act (U · mg⁻¹) with^a:		A₄₂₀/A₂₈₀	Yield (%)	Purification (fold)
Purification stage	DCIP^b	cyt c^c	A₄₂₀/A₂₈₀	Yield (%)	Purification (fold)
Supernatant	2.7	2.6	0.08	100	1
DEAE Sepharose	26	28	0.22	80	11
Resource PHE	63	66	0.51	55	25

Protein was determined using the Bradford protein assay. Using the BCA protein assay, the following results were obtained for pure CDH: DCIP activity, 16 U · mg⁻¹; cyt c activity, 18 U · mg⁻¹.

Activity was measured using DCIP and cellobiose at pH 4.0; this assay detects both CBQ and CDH.

Activity was measured using cyt c and cellobiose at pH 3.5; this assay detects only CDH.