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. 2001 Apr;67(4):1766–1774. doi: 10.1128/AEM.67.4.1766-1774.2001

TABLE 4.

Kinetic constants for competitive inhibition of lactose oxidation by its primary product, lactobiono-1,5-lactone, and by lactobionic acid or glucono-1,5-lactonea

Inhibitor Kinetic constants
Km (mM) Ki (mM) Km/Ki kcat (s−1)
Lactobionic acid, equilibrated 2.9 510 0.006 24
Ca-lactobionate 2.4 1,600 0.002 25
Lactobiono-1,5-lactone 2.6 31 0.08 25
Glucono-1,5-lactone 2.3 105 0.02 25
a

The electron acceptor used was DCIP.