TABLE 5.
A comparison of apparent kinetic constants for CDH and CBQ from S. rolfsii
Substrate or electron acceptor | Kinetic constants fora:
|
|||||
---|---|---|---|---|---|---|
CDH
|
CBQ
|
|||||
Km (mM) | kcat (s−1) | kcat/Km (mM−1 · s−1) | Km (mM) | kcat (s−1) | kcat/Km (mM−1 · s−1) | |
Cellobiose | 0.12 | 27 | 225 | 0.090 | 48 | 530 |
DCIP | 0.015 | 30 | 2,000 | 0.018 | 49 | 2,700 |
Kinetics were measured using cellobiose and DCIP at pH 4.0 and 25°C; protein was determined using the BCA method.