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. 2001 Apr;67(4):1766–1774. doi: 10.1128/AEM.67.4.1766-1774.2001

TABLE 5.

A comparison of apparent kinetic constants for CDH and CBQ from S. rolfsii

Substrate or electron acceptor Kinetic constants fora:
CDH
CBQ
Km (mM) kcat (s−1) kcat/Km (mM−1 · s−1) Km (mM) kcat (s−1) kcat/Km (mM−1 · s−1)
Cellobiose 0.12 27 225 0.090 48 530
DCIP 0.015 30 2,000 0.018 49 2,700
a

Kinetics were measured using cellobiose and DCIP at pH 4.0 and 25°C; protein was determined using the BCA method.