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. 2022 Jul 13;13:4071. doi: 10.1038/s41467-022-31742-1

Fig. 3. Genome-wide distribution of BDF5 determined by ChIP-seq analysis.

Fig. 3

Outer circles: Circos plot representing the 32 MB L. mexicana genome. The 34 chromosomes are depicted by grey segments. Inner circles represent results from BDF5 ChIP-seq. Formaldehyde-crosslinked, enzymatically fragmented chromatin from the BDF5::6xHA−/+flx strain was immunoprecipitated using anti-HA, reverse crosslinked and Illumina sequenced. Regions enriched >0.5 log2fold for BDF5 are indicated by the orange bars, the enrichment of BDF5::6xHA in the elution over the input chromatin is indicated in the blue line on a log2 fold scale, values are the mean derived from 3 ChIP replicates. Genomic features such as strand-switch regions (SSR), tRNA genes, and rRNA genes are indicated by coloured circles on this blue line. Inner panel: Key and stacked bar chart showing the genomic features associated with the peaks.