Table 1.

HPLC Cfm related substances impurities profile data after six hours of PCD via α-HNPs.

Impurity	IUPAC Name	Chemical name	Molecular formula	Molar mass (g/mol)	Rt (min)	Degradation (%)
A	(6R,7R)-7-((Z)-2-(2-aminothiazol-4-yl)-2-((methoxy-d3)imino)acetamido)-3-(methoxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylicacid	Cefetamet	C14H15N5O5S2	397.43	9.601	1.32
B	(6R,7R)-7-[[(2Z)-2-(2-aminothiazol-4-yl)-2-(methoxyimino)acetyl]amino]-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo [4.2.0]oct-2-ene-2-carboxylic acid	Deacetyl Cefotaxime	C14H15N5O6S2	413.43	2.934	0.27
C	(6R,7R)-3-[(acetyloxy)methyl]-7-[[(2Z)-2-[2-(formylamino)thiazol-4-yl]-2-(methoxyimino)acetyl]amino]-8-oxo-5-thia-1-azabicyclo [4.2.0]oct-2-ene-2-carboxylic acid	N-Formylcefotaxime	C17H17N5O8S2	483.48	4.458	1.36
E	(5aR,6R)-6-[[(2Z)-2-(2-aminothiazol-4-yl)-2-(methoxyimino)acetyl]amino]-5a,6-dihydro-3H,7H-azeto[2,1-b]furo[3,4-d][1,3]thiazine-1,7(4H)-dione	Deacetylcefota-xime Lactone	C14H13N5O5S2	395.41	8.272	1.00
F	(6R,7R)-3-[(acetyloxy)methyl]-7-[[(2Z)-2-[2-[[[(6R,7R)-7-[[(2Z)-2-(2-aminothiazol-4-yl)-2-(methoxyimino)acetyl]amino]-2-carboxy-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-en-2-yl]methyl]amino]thiazol-4-yl]-2-(methoxyimino)acetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid (	Cefotaxime Dimer	C30H30N10O12S4	850.88	14.649	0.14