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. 2022 Jun 15;26(14):3977–3994. doi: 10.1111/jcmm.17434

FIGURE 3.

FIGURE 3

DAPT administration extends the lifespan of stem cells. Cell cultures derived from holoclones and meroclones obtained from donor limbus and EEC‐OMESCs were treated with DAPT. The number of passages in the absence (red columns) or presence (blue columns) of DAPT (A). Significant increase in the number of passages after DAPT treatment of EEC‐OMESCs (B). Clonogenic cells, final cell number and ∆Np63α expression quantified by qPCR from holoclones (C–E), meroclones (F–H) and R279 H‐OMESCs (I–K) either untreated or treated with DAPT. Data are shown as mean ± standard deviation along with the results of statistical significance as calculated by ordinary one‐way anova with multiple comparisons (*p < .05; **p < .005; ***p < .0005; ****p < .0001). EEC‐OMESCs: ectrodactyly‐ectodermal dysplasia‐clefting oral mucosa epithelial stem cells; DAPT: N‐[N‐(3, 5‐difluorophenacetyl)‐l‐alanyl]‐S‐phenylglycine t‐butyl ester