Skip to main content
. 2001 Apr;67(4):1775–1782. doi: 10.1128/AEM.67.4.1775-1782.2001

TABLE 1.

Scatter and fluoresence values for total, HNA, and LNA cells as determined after staining with different nucleic acid dyes (SYTO 13, SYBR I, and SYBR II)a

Nucleic acid stain Mean scatter
Mean fluorescence
Total cell value HNA cell value LNA cell value Ratio of HNA cell value to LNA cell value Total cell value HNA cell value LNA cell value Ratio of HNA cell value to LNA cell value
SYTO 13 0.024 ± 0.006 0.025 ± 0.008 0.016 ± 0.003 1.61 ± 0.46 0.184 ± 0.034 0.262 ± 0.048 0.056 ± 0.011 4.75 ± 0.84
SYBR I 0.022 ± 0.005 0.024 ± 0.007 0.015 ± 0.003 1.63 ± 0.43 0.252 ± 0.025 0.329 ± 0.033 0.079 ± 0.014 4.32 ± 0.96
SYBR II 0.024 ± 0.007 0.026 ± 0.008 0.016 ± 0.003 1.72 ± 0.45 0.154 ± 0.025 0.210 ± 0.026 0.043 ± 0.010 4.88 ± 1.36
a

Means ± standard deviations were determined for samples collected at different times at the SOLA station (n = 16). The scatter and fluorescence values were normalized with fluorescent beads.