TABLE 1.
Nucleic acid stain | Mean scatter
|
Mean fluorescence
|
||||||
---|---|---|---|---|---|---|---|---|
Total cell value | HNA cell value | LNA cell value | Ratio of HNA cell value to LNA cell value | Total cell value | HNA cell value | LNA cell value | Ratio of HNA cell value to LNA cell value | |
SYTO 13 | 0.024 ± 0.006 | 0.025 ± 0.008 | 0.016 ± 0.003 | 1.61 ± 0.46 | 0.184 ± 0.034 | 0.262 ± 0.048 | 0.056 ± 0.011 | 4.75 ± 0.84 |
SYBR I | 0.022 ± 0.005 | 0.024 ± 0.007 | 0.015 ± 0.003 | 1.63 ± 0.43 | 0.252 ± 0.025 | 0.329 ± 0.033 | 0.079 ± 0.014 | 4.32 ± 0.96 |
SYBR II | 0.024 ± 0.007 | 0.026 ± 0.008 | 0.016 ± 0.003 | 1.72 ± 0.45 | 0.154 ± 0.025 | 0.210 ± 0.026 | 0.043 ± 0.010 | 4.88 ± 1.36 |
Means ± standard deviations were determined for samples collected at different times at the SOLA station (n = 16). The scatter and fluorescence values were normalized with fluorescent beads.