Figure 2.

Structure and physico-chemical characterization of TF-TCB. (A) Schematic diagram of TF-TCB structure. (B) Size exclusion-high performance liquid chromatography (SE-HPLC) analysis of TF-TCB: the major peak (11.7 min, 96.7%) was the TF-TCB, the much smaller peak (10.1 min, 3.33%) might be the aggregates. (C) SDS-PAGE analysis of TF-TCB. (D) Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC–QTOF-MS) analysis of the molecular weight of intact and deglycosylated TF-TCB. (E) Differential scanning calorimetry (DSC) thermogram and corresponding T_m values of TF-TCB and TF-011. (F) Binding kinetics and affinities of TF-TCB and TF-011, measured by Biacore. (G) Binding affinity of TF-TCB to CD3⁺ Jurkat cells. (H) Binding affinities of TF-TCB and TF-011 to TF⁺ MDA-MB-231 cells. MFI, mean fluorescence intensity of tested cells. Data are mean ± SD, n = 3.