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. 2021 Oct 22;12(4):2103–2119. doi: 10.1016/j.apsb.2021.10.010

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Intratumoral activation of immune response and immunologic mechanisms. (A) Quantitative analysis of the expression of TNF-α, IFN-γ, IL-1β in tumors from mice with different treatments by enzyme-linked immunosorbent assay (ELISA, n = 8). (B) Quantitative analysis of the expression of TNF-α, IFN-γ, IL-1β in serum collected from mice with different treatments by enzyme linked immunosorbent assay (ELISA, n = 8). (C) Representative image of immunohistochemical staining of the tumor slices. Analysis of the expression of HIF-1α, PD-L1, HMGA1, MPO and CD68 in tumor sections from all groups. Scar bar = 100 μm. Data are given as mean ± SD. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.