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. 2021 Oct 30;12(4):1913–1927. doi: 10.1016/j.apsb.2021.10.025

Figure 6.

Figure 6

PHF6 and SUV39H1 coordinatively regulate cell growth and proliferation in an interdependent manner. (A) The proliferation of Jurkat cells with the treatments indicated was monitored using a CCK-8 assay (n = 3). Immunoblots of PHF6 and SUV39H1 in the indicated Jurkat cells. (B) qPCR analysis of 47S pre-rRNA from (A) cells (n = 3). (C) The proliferation of U937 cells with the treatments indicated was monitored using a CCK-8 assay (n = 3). Immunoblots of PHF6 and SUV39H1 in the indicated U937 cells. (D) qPCR analysis of 47S pre-rRNA from (C) cells (n = 3). (E)–(G) Xenograft tumor growth of U937 AML cells infected with shNC, shPHF6, and over-PHF6 lentivirus (n = 5). Immunoblot of PHF6 in the indicated tumors. GAPDH was used as loading control. Scale bar, 10 mm. (H) H&E and ki67 staining for U937 xenograft tissues. Scale bar, 100 μm. (I) qPCR analysis of 47S pre-rRNA from U937 xenograft tissues (n = 5). (J) qPCR analysis of 47S pre-rRNA of PBMCs from AML patients (WT-PHF6 = 10 and Mutant-PHF6 = 5). All values are expressed as the mean ± SD; ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.