Figure 1.

Screening of Aβ binding components in KXS by the combinational use of BLI and UHPLC−DAD-Q/TOF-MS/MS. (A) Real-time kinetic binding sensorgrams of different concentrations of KXS increasing from 125 to 4000 μg/mL are shown. Response (nm) indicates the optical thickness on the SA biosensor layer. The equilibrium binding signal (Req) revealed by the flattened curve is reached. (B) The TICs were recorded by UHPLC−DAD-Q/TOF-MS/MS. S1: The dissociation buffer without KXS extract; S2: The dissociation buffer with KXS extract; S3: KXS extract used for the BLI assay. (C) The extracted ion chromatograms (EICs) of dehydrotumulosic acid (DTA) in S1, S2, and S3. (D) The EICs of tumulosic acid (TA) in S1, S2, and S3. (E) The EICs of polyporenic acid C (PPAC) in S1, S2, and S3.