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. 2022 Apr 4;93(7):761–771. doi: 10.1136/jnnp-2021-328710

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© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See: https://creativecommons.org/licenses/by/4.0/.

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Comparison of monoclonal and polyclonal anti-poly(GP) antibodies in Simoa homebrew assays. Homebrew Simoa assay conditions were optimised using different capture antibodies and detector antibodies (*). mGP=monoclonal poly(GP) antibody (TALS 828.179). GP57*−60* is a combination of two custom polyclonal antibodies ‘GP57’ and ‘GP60’. GP6834 is an alternative custom made poly(GP) antibody. Dashed lines show predicted LLOQs for each optimised assay respectively (mGP +mGP*, mGP +GP57*−60*, mGP +GP6834*), calculated using the Quanterix assay developer tool, after running 6-point standard curves using GST-GP32 as standard. AEB, average number of enzyme labels per bead; LLOQ, lower limit of quantification; Simoa, single molecule array.