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. 2022 Jun;10(12):683. doi: 10.21037/atm-22-2753

Figure 6.

Figure 6

BAMBI or SMAD6 silencing mimics the osteogenic roles of miR-20a in MC3T3-E1 cells. (A) Silencing efficiency was measured with qRT-PCR after MC3T3-E1 cells had been transfected with siRNAs targeting BAMBI or SMAD6, respectively. (B) ALP activity was enhanced by si-BAMBI, si-SMAD6, and miR-20a, respectively. (C) BMP2, RUNX2, and SP7 mRNA expressions were induced by si-BAMBI, si-SMAD6, and miR-20a, respectively. (D) Alizarin red S staining assay was conducted after the cells had been transfected with si-BAMBI, si-SMAD6, and miR-20a. Scale bar: 50 µm. Data are shown as mean ± SD for n=3; *, &, #, P<0.05; **, &&, ##, P<0.01; ***, ###, P<0.001 vs. NC. qRT-PCR, real-time quantitative polymerase chain reaction; ALP, alkaline phosphatase; BAMBI, BMP and activin membrane-bound inhibitor; SMAD6, mothers against decapentaplegic family member 6; si-BAMBI, silencing of BAMBI; si-SMAD6, silencing of SMAD6; NC, negative control group.