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. 2022 Jun 30;13:908449. doi: 10.3389/fimmu.2022.908449

Figure 2.

Figure 2

The orthotopic transplantation of APTAK organoids leads to both liver metastases and PC but only cancer cells in the peritoneal cavity show induction of senescence. (A) Schematic protocol of the orthotopic transplantation of APTAK organoids under the serosa of the cecum. (B) Quantification of primary tumors (98%, n = 27), liver metastases (54%, n = 15) and PC (57%, n = 16) about 42 days after orthotopic transplantation of APTAK organoids. (C) Representative images of IHC staining for the proliferation markers Cyclin D1 and Ki67, the DDR marker γ-H2AX, the senescence marker H3K9me3 and the stem cell marker Nanog in primary tumors (PT), liver metastases (LM) and PC. Scale bar: 50 µm (D) Gene set enrichment analysis (GSEA) of senescence and autophagy markers in PC versus primary tumor samples (n = 3 per group). (E) Representative images of tumor organoids cultured as 3D culture at d5 derived from tumors (primary tumor (PT), liver metastasis (LM) and peritoneal carcinomatosis (PC)) of the same mouse in the orthothopic organoid transplantation model. Magnification 20X.