Skip to main content
. 2022 Jun 30;13:908449. doi: 10.3389/fimmu.2022.908449

Figure 4.

Figure 4

Cancer cells from metastases in the peritoneal cavity of the APTAK mouse model show enhanced stem cell-like phenotype and a SASP. (A) Representative immunofluorescence (IF) images of the stem cell markers CD44, CD133 (green) and c-Myc (red) of tumor organoids derived from primary tumors (PT), liver metastases (LM) and PC from the orthotopic organoid transplantation CRC mouse model. The proliferation marker EdU stained in red and nuclei in blue. Scale bars represent 50 µm. (B) Quantification of IF markers from (A). 5 HPF with at least 1000 cells from primary tumors (n = 5), liver metastases (n = 3) and PC (n = 3) were counted. Error bars represent the mean ± SD. Kruskal-Wallis test (B) ****p < 0.0001, ***p < 0.0002, *p < 0.05, n.s. = not significant. (C) Heatmap of characteristic SASP genes from tumor organoids derived from primary tumors (PT), liver metastases (LM) and PC (n = 3 mice per group) from the orthotopic organoid transplantation CRC mouse model. Tumors were isolated, dissociated and cultured for one passage as 3D organoids. Then mRNA was isolated and qPCR was performed.