Table 6B.

Members of the chemokine signaling pathway showing differential phosphorylation between Salmonella enterica serovar Enteritidis-infected cecal tissue and non-infected cecal tissue: PI3K signaling pathway.

				4 hours pi		8 hours pi		24 hours pi
Functions	Peptide name	UniProt ID	p-site*	Fold change	p-value	Fold change	p-value	Fold change	p-value
Cytokine production Leukocyte migration Chemotaxis Actin cytoskeleton regulation Cellular shape change	PIK3R1	P27986	S608	1.907	0	1.107	0.014	–	–
	PIK3R2	O00459	Y612	1.670	0.006	1.521	0.036	–	–
	AKT1	P31749	S473	1.245	0.03	1.126	0.03	1.361	0
	CHUK (IκκA)	O15111	S194	1.086	0.017	–	–	1.262	0.002
	NFκB1A (IκB)	P25963	1.193 - 1.086	0.04 - 0.02	1.178 - -	0.01 - -	- - 1.262	- - 0.003	1.193 - 1.086
	NFκB-p105	P19838	S342	1.803	0.00001	1.233	0.004	–	–
	NFκB-p100	QOO653	S872	1.926	0	1.214	0.02	–	–
	ITK	Q08881	Y523	2.136	0	1.268	0.2	1.194	0.016
	CDC42	P60953	Y64	1.418	0	1.218	0.005	–	–
	WASP	P42786	Y256	1.484	0	1.272	0.04	–	–
	RhoA	P61586	S188	–	–	–	–	-1.174	0
	ROCK2	O75116	Y500	1.231	0.002	-1.266	0	-1.168	0.01
	FAK	Q05397	Y397	1.220	0.001	-1.263	0	-1.143	0.001
	CRK	P46108	Y252	1.806	0.001	–	–	-1.402	0
	PYK2	Q14289	Y405	1.259	0.001	–	–	-1.402	0
	PAK1	Q13153	T212	1.442	0.019	1.275	0.04	–	–
	RAC1	P63000	S71	1.252	0	1.23	0	1.314	0

Selection of peptides differentially phosphorylated between non-infected group and S. Enteritidis challenge group in the cecum. The UniProt ID identifies the protein, while the p-site identifies the specific phosphorylation target site on that protein. Fold-Change indicates the directionality of phosphorylation status for each treatment compared to control. The p-value is the measure of significance (α = 0.05). UniProt IDs and p-site correspond to human proteins for annotation purposes.

*= Phosphorylation site.