Skip to main content
. 2022 Jun 11;36(7):e24514. doi: 10.1002/jcla.24514

FIGURE 2.

FIGURE 2

ALKBH5 may drive HPSCC cell ferroptosis. (A) m6A dot blot assay of the global m6A abundance in mRNAs of control and shALKBH5HPSCC cells. MB, methylene blue staining (as a loading control). (B) Western blot analysis of the indicated ferroptosis‐related proteins in control and shALKBH5 Detroit 562 cells. β‐Actin was used as the loading control. (C) Representative immunofluorescence staining of GPX4 (scale bar = 200 μm, magnification: E, 20×) in control and shALKBH5 Detroit 562 cells 24 h after RSL3 treatment. (D) Cell viability assay of control and shALKBH5 Detroit 562 cells 24 h after RSL3 treatment (scale bar = 200 μm, magnification: E, 20×). (E) LDH release assay of control and shALKBH5 Detroit 562 cells 24 h after RSL3 treatment. (F) Measurement of lipid ROS levels in control and shALKBH5 Detroit 562 cells by BODIPY C11 staining after exposure to RSL3 (5 μM) for 24 h. (G) Ultrastructural analysis of shCON and shALKBH5 cells. The red arrows indicate outer membrane rupture (scale bar = 500 nm)