FIGURE 5.

BHLHE40 directly regulates INHBA expression. (A) Western blot showed that BHLHE40 protein levels in the colon cancer cells (HCT116, SW480, SW620) were remarkably elevated in contrast with normal intestinal epithelial cells (CCD 841 CoN). (B) INHBA expression was suppressed by silencing BHLHE40 expression in colon cancer cells. (C) ChIP showed that BHLHE40 might bind to the promoter region of INHBA. Data are shown as mean ± SD of three independent experiments. *, **, and *** indicate p = < 0.05, <0.01, and <0.001, respectively