FIGURE 3.

Azelastine is effective against SARS-CoV-2 infection in Vero cell lines. (A) Vero E6 or (B) Vero-TMPRSS2/ACE2 cells were infected with SARS-CoV-2 B.1.177 simultaneously with or 30 min prior to the addition of 0.4–12.5 µM of azelastine. After 48 h, RNA was extracted from the cell culture supernatant and was quantified by droplet digital PCR (A) or qPCR (B) analysis. Graphs show inhibition of infection expressed as viral genome count relative to untreated, virus only control (%) and the mean ± SEM from 5 (A) or 3 (B) biological replicates and 2 (A) or 3 (B) technical repeats.