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. 2022 Jul 12;154(8):e202213164. doi: 10.1085/jgp.202213164

Figure 1.

Figure 1.

TMEM120A inhibits PIEZO2 currents. Whole-cell patch-clamp experiments at −60 mV in cells transiently transfected with Piezo2 with or without Tmem120a were performed as described in the Materials and methods section. (A) HEK293 cells were transfected with Piezo2 and GFP with or without Tmem120a. In some cells, GFP-tagged Piezo2 was used instead of Piezo2 plus GFP. Current amplitudes are plotted (mean ± SEM) for Piezo2 expressing cells (black) and for cells expressing Piezo2 and Tmem120a (red). (B) Scatter plots and mean ± SEM for current amplitudes at 4.8 μm indentation. Statistical significance was calculated with the Mann-Whitney test. (C) Representative current traces. (D) HEK293 cells were transfected with GFP-tagged Piezo2 and with tdTomato-tagged Tmem120a or tdTomato. Current amplitudes are plotted (mean ± SEM) for Piezo2 expressing cells (black) and for cells expressing Piezo2 and Tmem120a (red). (E) Scatter plots and mean ± SEM for current amplitudes at 4.4 μm indentation. Statistical significance was calculated with the Mann-Whitney test. (F) Representative current traces. (G) Piezo1 deficient N2A cells were transfected with GFP-tagged Piezo2 and with tdTomato-tagged Tmem120a or tdTomato. Current amplitudes (mean ± SEM) are plotted for Piezo2 expressing cells (black) and for cells expressing Piezo2 and Tmem120a (red). (H) Scatter plots and mean ± SEM for current amplitudes at 6.4 μm indentation. Statistical significance was calculated with the Mann-Whitney test. (I) Representative current traces.