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. 2022 Jul 12;154(8):e202213164. doi: 10.1085/jgp.202213164

Figure S2.

Figure S2.

TMEM120A does not inhibit PIEZO1 and TREK1 currents. Data from Fig. 2 showing the full range of measurements with indentation depth or negative pressure increased until the seal was lost. (A and B) Data from whole-cell patch-clamp experiments in HEK293 cells transfected with Piezo1 alone or Tmem120a + Piezo1. (C and D) Data from cell-attached patch clamp experiments in Piezo1 deficient N2A cells transfected with Piezo1 alone or Tmem120a + Piezo1. (E) Representative traces for cell-attached patch clamp experiments in N2A cell transfected with tdTomato-Tmem120a. (F) Full range of data from cell-attached patch-clamp experiments in N2A cell transfected with tdTomato-Tmem120a. (G) Representative traces for cell-attached patch-clamp experiments in N2A cell transfected with tdTomato. (H) Full range of data from cell-attached patch-clamp experiments in N2A cells transfected with tdTomato. (I–L) Full range of data from cell-attached patch-clamp experiments at 0 mV in N2A cells transfected with TREK1 + tdTomato (I), TREK1 + tdTomato-Tmem120a (J), tdTomato-Tmem120a alone (K), and tdTomato alone (L). The slight discrepancy in the number of cells compared to Fig. 2 in C, D, F, and I–L is because these panels include cells where the seal was lost before the negative pressure reached 55 mmHg, and those cells were excluded from Fig. 2.