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. 2022 Jun 30;13:927955. doi: 10.3389/fimmu.2022.927955

Figure 2.

Figure 2

Outer membrane protein A (OmpA)+ A. baumannii increased pulmonary permeability and promoted epithelial barrier dysfunction. (A) Evans blue dye (EB) permeability through the pulmonary epithelium of uninfected (Sham) and different A. baumannii strain-infected C57BL/6J mice measured in bronchial alveolar lavage fluid (BALF) (n = 3 per group). (B) EB-conjugated albumin (EBA, final concentration: 0.67 mg/mL) flux after infection with different A. baumannii strains (MOI = 50). (C, D) TEER of A549 cell monolayers at multiple time points after treatment with different A. baumannii strains. (E) Purified OmpA pre-treatment affected the EBA permeability and (F) TEER of A549 cells at multiple time points. Data are from three independent experiments; error bars represent standard deviation. **P<0.01,*P<0.05 vs. Control (or Sham, blank) group, ## P<0.01, # P<0.05.