Figure 2.

Sequence features of TcCAL1 and a phylogenetic tree. (A) Amino acid sequence and position of the EF-hand motifs. Residues in black and red bold indicate the alpha helices and the Ca²⁺ binding cleft, respectively. The arrow indicates the phosphorylated serine. (B) Predicted tridimensional structure of TcCAL1. The circles represent Ca²⁺ ions. The different color intensity indicates the model confidence, being high at the C-terminus and lower at the N-terminus (dark and light blue, respectively). (C) TcCAL1 evolutionary relationship with different species of trypanosomatids. The scale-bar represents 0.3 substitutions per position in the aminoacid sequence. The numbers at the branches indicate bootstrap values. TcCAL1 is highlighted in bold.