TABLE 2.
Expression of AFP1, CIRP, YB-1, and HMGB1 in different tissues from fish cultured at different temperatures (means ± standard deviations).
| Tissue | Gene | Expression | |||
|---|---|---|---|---|---|
| 5°C | 8°C | 13°C | 18°C | ||
| Brain | AFP1 | 1066.966 ± 58.627 | 684.934 ± 160.236 | 155.071 ± 10.673 | 33.485 ± 3.920 |
| CIRP | 19.983 ± 8.395 | 7.417 ± 2.371 | 18.696 ± 6.166 | 7.302 ± 1.900 | |
| HMGB1 | 86.856 ± 33.051 | 684.411 ± 91.780 | 104.362 ± 7.083 | 28.043 ± 2.521 | |
| YB-1 | 35.377 ± 7.525 | 2.077 ± 0.299 | 29.748 ± 6.091 | 14.051 ± 0.647 | |
| AFP1 | 6293.382 ± 351.457 | 1499.415 ± 286.439 | 433.506 ± 50.107 | 40.844 ± 5.496 | |
| Heart | CIRP | 36.390 ± 5.614 | 46.828 ± 15.878 | 12.978 ± 2.688 | 12.447 ± 2.165 |
| HMGB1 | 30.119 ± 3.246 | 371.701 ± 94.342 | 19.716 ± 2.299 | 16.091 ± 1.397 | |
| YB-1 | 181.004 ± 50.614 | 26.619 ± 3.228 | 111.872 ± 13.355 | 71.776 ± 11.511 | |
| AFP1 | 1282.112 ± 113.425 | 1293.809 ± 287.443 | 313.135 ± 27.137 | 49.393 ± 1.630 | |
| Intestine | CIRP | 7.494 ± 1.168 | 12.797 ± 0.624 | 8.024 ± 0.397 | 1.012 ± 0.193 |
| HMGB1 | 3.419 ± 0.285 | 70.688 ± 10.000 | 13.614 ± 0.943 | 1.002 ± 0.093 | |
| YB-1 | 19.524 ± 4.315 | 5.130 ± 3.108 | 32.797 ± 4.534 | 4.633 ± 0.599 | |
| AFP1 | 2619.441 ± 138.213 | 2607.893 ± 763.005 | 273.210 ± 43.607 | 138.641 ± 18.764 | |
| Kidney | CIRP | 13.113 ± 2.094 | 7.594 ± 1.847 | 19.429 ± 1.665 | 6.787 ± 0.517 |
| HMGB1 | 7.102 ± 0.812 | 69.846 ± 7.990 | 16.645 ± 0.372 | 4.534 ± 0.509 | |
| YB-1 | 16.438 ± 4.316 | 1.232 ± 1.149 | 50.748 ± 8.688 | 9.875 ± 0.626 | |
| AFP1 | 1794.711 ± 268.371 | 489.156 ± 39.355 | 365.993 ± 88.821 | 57.209 ± 29.476 | |
| Liver | CIRP | 13.519 ± 7.766 | 5.962 ± 1.397 | 21.564 ± 6.086 | 9.331 ± 2.064 |
| HMGB1 | 18.601 ± 1.117 | 89.556 ± 13.502 | 96.255 ± 10.374 | 17.639 ± 0.471 | |
| YB-1 | 28.098 ± 1.714 | 5.407 ± 1.882 | 68.932 ± 7.887 | 53.148 ± 8.477 | |
| AFP1 | 6116.457 ± 1378.037 | 1811.152 ± 690.646 | 387.966 ± 27.999 | 194.620 ± 36.069 | |
| Muscle | CIRP | 89.788 ± 57.712 | 40.804 ± 11.521 | 16.792 ± 1.359 | 15.674 ± 0.434 |
| HMGB1 | 66.614 ± 5.001 | 121.446 ± 5.530 | 50.482 ± 1.620 | 35.631 ± 3.744 | |
| YB-1 | 3514.809 ± 638.609 | 509.614 ± 165.296 | 2000.654 ± 86.534 | 2710.596 ± 483.487 | |
| AFP1 | 3512.656 ± 31.531 | 3179.010 ± 33.714 | 388.093 ± 49.514 | 91.363 ± 9.527 | |
| Spleen | CIRP | 19.201 ± 3.057 | 12.285 ± 4.973 | 5.310 ± 0.349 | 3.982 ± 0.129 |
| HMGB1 | 15.677 ± 1.745 | 101.118 ± 21.340 | 9.094 ± 0.752 | 4.701 ± 0.398 | |
| YB-1 | 29.009 ± 2.606 | 0.225 ± 0.148 | 20.453 ± 2.186 | 5.817 ± 1.006 | |
| AFP1 | 7400.707 ± 868.984 | 893.144 ± 54.822 | 441.387 ± 55.797 | 145.049 ± 11.767 | |
| Skin | CIRP | 15.086 ± 0.933 | 18.135 ± 0.696 | 16.181 ± 4.398 | 4.378 ± 0.133 |
| HMGB1 | 11.748 ± 0.850 | 59.467 ± 5.569 | 29.777 ± 3.075 | 13.118 ± 2.621 | |
| YB-1 | 151.941 ± 40.267 | 66.343 ± 14.264 | 66.961 ± 8.481 | 75.281 ± 5.565 | |
| AFP1 | 2933.386 ± 245.377 | 449.5484 ± 128.829 | 4.213 ± 0.471 | 1.025 ± 0.286 | |
| Gonad | CIRP | 9.144 ± 0.729 | 7.027 ± 1.484 | 2.295 ± 0.246 | 2.733 ± 0.260 |
| HMGB1 | 22.158 ± 3.001 | 35.308 ± 4.753 | 3.276 ± 0.243 | 3.623 ± 0.611 | |
| YB-1 | 21.180 ± 4.578 | 1.818 ± 0.545 | 2.847 ± 0.326 | 1.004 ± 0.116 | |