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. 2022 Jun 30;9:897935. doi: 10.3389/fmolb.2022.897935

TABLE 3.

Split-split-plot analysis of variance for T. rubripes cold resistant experiment with four cold resistant genes, four temperature gradients and nine tissues.

Source of Variation Sum of Square Degrees of Freedom Mean Square F-Value p-value
Blocks (replicates) 40252485 5 8050497
Temperature 29812447 3 9937482.5 4.9102333* 0.0143
Main-plot error 30357466 15 2023831
Tissue 17330089 8 2166261.1 9.1797** 0
Temperature × tissue 17897111 24 745712.94 3.16** 0
Split-plot error 37757296 160 235983.1
Gene 65742384 3 21914128 52.140,554** 1E-07
Temperature × gene 80367779 9 8929753.2 21.246,671** 1E-07
Tissue × gene 30951837 24 1289659.9 3.0685,036** 1.887E-06
Temperature × tissue ×gene 43351378 72 602102.48 1.4325898* 0.0152,694
Split-split-plot error 226956339 540 420289.52

Notes: Asterisks denote that correlations were significant at *p < 0.05 and **p < 0.01.