TABLE 4.
Results of AMMI analysis of expression of the four genes in different tissues from fish cultured at different temperatures.
| Source of | 5°C | |||||
|---|---|---|---|---|---|---|
| Variation | df | SS | MS | F | Prob | % Of Total SS |
| Total | 107 | 423623818.3 | 3959101.1 | |||
| Treatment | 35 | 416897702.1 | 11911363 | 127.51** | 0 | |
| Gene | 3 | 251594453.7 | 83864818 | 897.73** | 0 | 59.3910 |
| Tissue | 8 | 56355829.37 | 7,044,478.7 | 75.408** | 0 | 13.3032 |
| Interaction | 24 | 108,947,419.1 | 4,539,475.8 | 48.593** | 0 | 25.7179 |
| IPCA1 | 10 | 90,456,102.53 | 9,045,610.3 | 96.829** | 0 | 83.0273 |
| IPCA2 | 8 | 18,486,274.89 | 2,310,784.4 | 24.736** | 0 | 16.9680 |
| Residual | 6 | 5041.6457 | 840.2743 | |||
| Error | 72 | 6,726,116.19 | 93418.28 | |||
| Source of | 8 °C | |||||
| variation | df | SS | MS | F | Prob | % of total SS |
| Total | 107 | 63,381,780.3 | 592353.087 | |||
| Treatment | 35 | 60,745,799 | 1,735,594.26 | 47.406** | 0 | |
| Gene | 3 | 37,053,112.4 | 12351037.5 | 337.36** | 0 | 58.460 |
| Tissue | 8 | 5,634,930.89 | 704366.361 | 19.239** | 0 | 8.890 |
| Interaction | 24 | 18,057,755.7 | 752406.488 | 20.551** | 0 | 28.490 |
| IPCA1 | 10 | 16914065.4 | 1,691,406.54 | 46.199** | 0 | 93.666 |
| IPCA2 | 8 | 938525.287 | 117315.661 | 3.204** | 0.003 | 5.197 |
| Residual | 6 | 205165.014 | 34194.169 | |||
| Error | 72 | 2,635,981.37 | 36610.852 | |||
| Source of | 13 °C | |||||
| variation | df | SS | MS | F | Prob | % of total SS |
| Total | 107 | 12,642,216.3 | 118152 | |||
| Treatment | 35 | 12,586,769.2 | 359622 | 466.982** | 0 | |
| Gene | 3 | 1,859,582.52 | 619861 | 804.911** | 0 | 14.709 |
| Tissue | 8 | 3,013,081.06 | 376635 | 489.074** | 0 | 23.833 |
| Interaction | 24 | 7,714,105.65 | 321421 | 417.377** | 0 | 61.018 |
| IPCA1 | 10 | 7,373,491.72 | 737349 | 957.474** | 0 | 95.584 |
| IPCA2 | 8 | 328220.754 | 41027.6 | 53.275** | 0 | 4.254 |
| Residual | 6 | 12393.176 | 2065.53 | |||
| Error | 72 | 55447.070 | 770.098 | |||
| Source of | 18 °C | |||||
| variation | df | SS | MS | F | Prob | % of total SS |
| Total | 107 | 21594750.1 | 201820 | |||
| Treatment | 35 | 21,121,069.5 | 603459 | 91.726** | 0 | |
| Gene | 3 | 1,829,837.74 | 609946 | 92.712** | 0 | 8.473 |
| Tissue | 8 | 5,404,080.57 | 675510 | 102.678** | 0 | 25.024 |
| Interaction | 24 | 13,887,151.2 | 578631 | 87.952** | 0 | 64.308 |
| IPCA1 | 10 | 13,847,644.7 | 1,384,764 | 210.486** | 0 | 99.715 |
| IPCA2 | 8 | 38976.135 | 4872.02 | 0.740 | 0.655 | 0.280 |
| Residual | 6 | 530.417 | 88.4028 | |||
| Error | 72 | 473680.549 | 6578.9 | |||
Notes: one df: degrees of freedom, SS: sum of squares, MS: mean squares, F: test statistic, Prob: probability.
2 **: significant at 1% probability level.