FIGURE 2.

EBL exerted the inhibitory effect on influenza A/PR/8/34-GFP viral infection in a dose-dependent manner. EBL (0, 10, 100, or 200 μg/ml) was mixed with PR8-GFP IAV for 1 h at 4°C. The mixtures were added to RAW 264.7 cells for 2 h at 37°C. After washing with PBS, the cells were further incubated for 24 h. (A) Brightfield and fluorescence images were captured with the fluorescent microscope at a ×200 magnification. (B) After fixing with 4% paraformaldehyde and resuspended in PBS, the cells were analyzed for GFP expression by FACS. (C) Levels of GFP expression were depicted as relative intensities compared to those of the control PR8-GFP IAV. The data represent the mean ± SD based on three independent experiments. Statistical significance was assessed via an unpaired Student T-test. ***p < 0.0001, **p < 0.001, and *p < 0.005.