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Electrophoretic analysis of X-PDP active fractions obtained at different purification steps. (A) SDS-PAGE; (B) native PAGE. Lane 1, molecular weight markers (positions indicated in kilodaltons); lane 2, cell extract; lane 3, Phenyl-Sepharose chromatography; lane 4, Resource Q chromatography (0 to 0.3 M NaCl); lane 5, Biosep-DEAE chromatography; lane 6, Sephacryl 200 HR chromatography; lane 7, Resource Q chromatography (0.1 to 0.25 M NaCl).
