TABLE 1.

Purification of X-PDP from L. sakei

Purification step	Total protein (mg)	Total activity (U)	Sp act (U/mg)	Yield (%)	Purification (fold)
Cell extract	1,137.50	22,563.8	19.8	100	1.0
50–70% ammonium sulfate cut	392.50	20,216.6	51.5	89.6	2.6
Phenyl-Sepharose	54.89	16,262.3	296.3	72.1	15.0
Resource Q (0–0.3 M NaCl)	4.74	9,636.4	2,033.1	42.7	102.7
Biosep-DEAE	1.74	5,128.3	2,947.3	22.7	148.8
Sephacryl 200 HR	0.52	2,827.8	5,438.1	12.5	274.6
Resource Q (0.1–0.25 M NaCl)	0.11	1,560.7	14,188.2	6.9	716.6