TABLE 2.
Effects of chemical agents on the activity of purified (615.1-fold) X-PDP
Chemical agent | Relative activitya
|
|
---|---|---|
0.1 mM | 1.0 mM | |
EDTA | 104 | 101 |
o-Phenanthroline | 104 | 103 |
Dithiothreitol | 106 | 105 |
2-Mercaptoethanol | 103 | 102 |
Iodoacetate | 107 | 89 |
E-64 | 91 | 112 |
Pepstatin A | 100 | 113 |
Amastatin | 96 | 100 |
Bestatin | 93 | 98 |
Puromycin | 89 | 94 |
Phenylmethylsulfonyl fluoride | 90 | 57 |
3,4-Dichloroisocoumarin | 0 | 0 |
Expressed as a percentage of that obtained in the absence of any added chemical agent, which was given a value of 100%, corresponding to 0.014 U (μmol · h−1).