TABLE 2.
Effects of chemical agents on the activity of purified (615.1-fold) X-PDP
| Chemical agent | Relative activitya
|
|
|---|---|---|
| 0.1 mM | 1.0 mM | |
| EDTA | 104 | 101 |
| o-Phenanthroline | 104 | 103 |
| Dithiothreitol | 106 | 105 |
| 2-Mercaptoethanol | 103 | 102 |
| Iodoacetate | 107 | 89 |
| E-64 | 91 | 112 |
| Pepstatin A | 100 | 113 |
| Amastatin | 96 | 100 |
| Bestatin | 93 | 98 |
| Puromycin | 89 | 94 |
| Phenylmethylsulfonyl fluoride | 90 | 57 |
| 3,4-Dichloroisocoumarin | 0 | 0 |
Expressed as a percentage of that obtained in the absence of any added chemical agent, which was given a value of 100%, corresponding to 0.014 U (μmol · h−1).