TABLE 4.
Relative activity of purified (716.6-fold) X-PDP on various fluorescent (AMC derivatives) and colorimetric (pNA derivatives) substrates
| Substrate | Relative activitya |
|---|---|
| Gly-Pro-AMC | 100.0 |
| Gly-Arg-AMC | 0.0 |
| Ala-Arg-AMC | 0.0 |
| Arg-Arg-AMC | 0.0 |
| Pro-Arg-AMC | 0.0 |
| Gly-Gly-AMC | 0.0 |
| Gly-Ala-AMC | 0.4 |
| Lys-Ala-AMC | 9.9 |
| Ala-Ala-Phe-AMC | 0.0 |
| Arg-AMC | 0.0 |
| Gly-AMC | 0.0 |
| Leu-AMC | 0.0 |
| Pro-AMC | 0.0 |
| Ala-Pro-pNA | 100.0 |
| Arg-Pro-pNA | 60.5 |
| Gly-Pro-pNA | 25.4 |
| Ala-Ala-pNA | 1.7 |
a
Expressed as a percentage of the rates of hydrolysis of Gly-Pro-AMC and Ala-Pro-pNA, which were given a value of 100%, corresponding to 0.011 and 0.089 U (μmol h−1), respectively.