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. 2022 Mar 9;29(5):3838–3847. doi: 10.1016/j.sjbs.2022.03.011

Fig. 2.

Fig. 2

Chrysin and Capsaicin stimulates Mitochondrial ROS and induces premature senescence in HeLa cells. Change in Mitochondrial ROS production (A) Chrysin treatment (B) Capsaicin treatment compared with the Control. Mitochondrial Membrane Potential was measured after (C) Chrysin treatment (D) Capsaicin treatment. Increase in cellular size (FSC-A) and granularity (SSC-A) post (E) Chrysin and, (F) Capsaicin treatment demonstrating senescence induction. Cellular autofluorescence (AF) and histogram profile (G) Chrysin and, (H) Capsaicin treatment showing enhanced AF relative to the Control indicates senescence. (I) Senescence-associated β-galactosidase (SA–β-Gal) staining in cells treated with Chrysin and Capsaicin. (J) Senescence-associated heterochromatic foci (SAHF) detected by DAPI staining in cells treated with Chrysin 50 µM and Capsaicin 100 µM and nuclear fragmentation at Chrysin 100 µM and Capsaicin 200 µM concentration treatment.