Fig. 3.

Cell Cycle analysis and Immuno-cytochemical detection of activated apoptotic signaling upon nuclear translocation of P53 and NF-κB. (A) Cell cycle analysis shows an arrest in the G2/M phase with the increase in the G2 population in cells with treated Chrysin 100 µM concentration. (B) Cell cycle analysis shows G1/S phase arrest with the increase in the G1 population in cells treated with Capsaicin 200 µM concentration. Immuno-cytostaining showing (C) nuclear translocation of p53 and (D) NF-κB in both Chrysin and Capsaicin treated cells. (E) Histogram profile showing NF-κB Cytoplasmic and nuclear localization as denoted by dark shades and light shades respectively. (F) Histogram profile showing p53 Cytoplasmic and nuclear localization as denoted by dark shades and light shades respectively. The level of proteins was measured in 200 + cells. N = 3 ± SE (*P < 0.1; ^**P < 0.05 and ^***P < 0.01).