Table 4.
Precision and Accuracy Data for 5-Fluorouracil (5-FU).
| Intra–batch Inter–batch |
% Recovery | |||||||
|---|---|---|---|---|---|---|---|---|
| QC ID | Theoretical Content (ng mL−1) | Mean concentration observed (ng mL−1) | Accuracya (%) | CVb (%) | Mean concentration observed (ng mL−1) | Accuracya (%) | CVb (%) | |
| LOQQC | 1.02 | 0.99 ± 0.03 | 97.06 | 3.03 | 0.98 ± 0.04 | 96.08 | 4.08 | 78.28 ± 2.31 |
| LQC | 2.92 | 2.90 ± 0.06 | 99.32 | 2.07 | 2.87 ± 0.05 | 98.97 | 1.74 | 79.13 ± 2.02 |
| MQC | 415.00 | 410.03 ± 11.06 | 98.80 | 2.70 | 408.24 ± 10.25 | 98.37 | 2.51 | 81.87 ± 3.27 |
| HQC | 815.00 | 795.62 ± 21.03 | 97.62 | 2.64 | 791.54 ± 20.14 | 97.12 | 2.54 | 83.10 ± 4.11 |
Values (Mean ± SD) are derived from 6 replicates: aAccuracy (%) = Mean value of [(mean observed concentration)/(theoretical concentration)] × 100; bPrecision (%): Coefficient of variance (percentage) = standard deviation divided by mean concentration found × 100; Theoretical contents; LOQQC: 1.010 ng mL−1, LQC: 2.92 ng mL−1; MQC: 415.0 ngmL−1; and HQC: 815.0 ngmL−1.