Table 1.
Sterilant treatments for culture asepsis and explant survival of nodal explants of Winter Jasmine (Jasminum nudiflorum Lindl.).
| Treatment | Sterilant used | Concentration (%) | Treatment duration | Culture asepsis (%) | Explant survival (%) |
|---|---|---|---|---|---|
| T1 | Mercuric chloride | 0.1 | 10 min | 62.44 ± 0.67d | 52.00 ± 0.88c |
| T2 | Sodium Hypochlorite | 1.0 | 10 min | 59.23 ± 0.29e | 58.33 ± 0.29b |
| T3 | Mercuric chloride followed by | 0.1 | 10 min | 69.44 ± 1.31c | 61.11 ± 0.77ab |
| Ethyl alcohol | 70.0 | 10 s | |||
| T4 | Sodium hypochlorite followed by | 1.0 | 10 min | 63.88 ± 0.97d | 63.88 ± 2.78a |
| Ethyl alcohol | 70.0 | 10 s | |||
| T5 | Flusilazole followed by | 0.01 | 20 min | 72.22 ± 0.29b | 44.66 ± 0.58d |
| Mercuric chloride followed by | 0.1 | 10 min | |||
| Ethyl alcohol | 70 | 10 s | |||
| T6 | Flusilazole followed by | 0.01 | 20 min | 67.89 ± 0.87c | 54.44 ± 0.58c |
| Sodium hypochlorite followed by | 1.0 | 10 min | |||
| Ethyl alcohol | 70 | 10 s | |||
| T7 | Flusilazole followed by | 0.02 | 20 min | 77.78 ± 0.97a | 42.88 ± 1.13d |
| Mercuric chloride followed by | 0.1 | 10 min | |||
| Ethyl alcohol | 70 | 10 s | |||
| T8 | Flusilazole followed by | 0.02 | 20 min | 73.88 ± 0.17b | 51.99 ± 0.38c |
| Sodium hypochlorite followed by | 1.0 | 10 min | |||
| Ethyl alcohol | 70 | 10 s | |||
| C.D (P≤0.05) | 2.39 | 3.56 | |||
*Data noted at the end of 4 weeks of culture on MS medium.
Data are a mean of three replicates with ± SE. Same superscript letters in a column do not differ significantly when compared by Duncan’s MRT at 5% level of significance.