TABLE 2.
MRM-based mass spectrometry parameters for the detection of purine metabolites.
| Compounds | Molecular weight | Q1/Q3 | DP (V) | CE (eV) | Ion mode |
|---|---|---|---|---|---|
| AMP | 347.2 | 348.1–136.1 | 40 | 16 | + |
| IMP | 348.2 | 349.2–137.2 | 35 | 8 | + |
| GMP | 363.1 | 364.1–152.1 | 40 | 12 | + |
| Adenosine | 267.2 | 268.1–136.1 | 40 | 20 | + |
| Inosine | 268.2 | 269.1–137.1 | 21 | 20 | + |
| Xanthosine | 284.23 | 285.04–152.03 | 35 | 15 | + |
| Guanosine | 283.24 | 284.1–152.1 | 40 | 20 | + |
| Adenine | 135.1 | 136.0–119.0 | 36 | 20 | + |
| Hypoxanthine | 136.1 | 137.0–110.0 | 36 | 20 | + |
| Xanthine | 152.1 | 153.1–110.1 | 35 | 15 | + |
| Guanine | 151.1 | 152.0–110.0 | 46 | 10 | + |
| IS1 | 301.69 | 302.1–170.0 | 35 | 12 | + |
| IS2 | 287.359 | 288.0–213.0 | 20 | 20 | + |