Table 9.
Relative quantification of injected bacterial strains 108 cell/plant in sour orange after two weeks.
| Treatments used for this study | Inoculation methods | Relative quantity of bacterial endophytes |
|---|---|---|
| Positive Control | Injected with ddH2O | 2.49 × 1014 ± 0.08 |
| Proteus mirabilis | Soil mixing | 7.74 × 106 ± 0.65 |
| Spray method | 3.19 × 108 ± 0.06 | |
| Injection method | 0* | |
| Bacillus cereus | Soil mixing | 0.25 × 104 ± 0.01 |
| Spray method | 0.38 × 105 ± 0.05 | |
| Injection method | 0* | |
| Bacillus safensis | Soil mixing | 0* |
| Spray method | 9.76 × 106 ± 0.07 | |
| Injection method | 0.35 × 106 ± 0.06 | |
| Bacillus megaterium | Soil mixing | 0.09 × 105 ± 0.22 |
| Spray method | 0.37 × 106 ± 0.11 | |
| Injection method | 75.69 × 106 ± 1.26 | |
| Staphylococcus haemolyticus | Soil mixing | 0.15 × 103 ± 0.07 |
| Spray method | 0.01 × 101 ± 0.02 | |
| Injection method | 0* | |
| Enterobacter hormaechei | Soil mixing | 0.05 × 105 ± 0.01 |
| Spray method | 0.02 × 102 ± 0.03 | |
| Injection method | 1.36 × 1014 ± 0.02 | |
| Enterococcus faecalis | Soil mixing | 4.13 × 108 ± 0.08 |
| Spray method | 2.56 × 106 ± 0.06 | |
| Injection method | 15.39 × 109 ± 1.21 | |
| Pseudomonas aeruginosa | Soil mixing | 0.62 × 104 ± 0.03 |
| Spray method | 0* | |
| Injection method | 10158.94 × 102 ± 1.56 | |
| Pseudomonas sp. | Injection method | 0* |
| Spray method | 1.53 × 105 ± 0.02 | |
| Soil mixing | 2.47 × 109 ± 0.05 | |
| Brevibacillus borstelensis | Soil mixing | 0.15 × 103 ± 0.06 |
| Spray method | 0.21 × 103 ± 0.03 | |
| Injection method | 0* | |
Note: Expression as x-fold rise in injected endophytic bacteria in treated sample in comparison to the healthy (mean of 3 replications, SD.
Below the detection limit of 54 copies per microlitre (µl).