Mutagenic evaluation of an essential oil mix of sweet orange peel, cumin, and allspice. The number of revertant colonies of S. typhimurium TA100 strain treated with different concentrations of essential oil mix (1.25, 2.5, and 5 mg/plate) in the absence of metabolic activation system (−S9). For the positive and negative control, 50 μg methyl methanesulfonate and 50 μl of pH 7.4 PBS were used. Values are expressed as mean ± SE of three independent experiments. Significantly different ∗∗∗∗p < 0.001 compared to the negative control.