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. 2022 Jun 30;16:881632. doi: 10.3389/fnint.2022.881632

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Copyright © 2022 Takiguchi, Miyashita, Yamazaki and Funakoshi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Images of Wisteria Floribunda agglutinin (WFA) histochemistry (green), and immunohistochemistry for NeuN (converted to blue), and 5-HT (red) in the L5 ventral horn of rats with saline (A,C,E,G,I,K), and ChABC (B,D,F,H,J,L) administration after 3 (A–F) and 8 (G–L) weeks after CST. Double-labeled images of 5-HT and NeuN (A,B,G,H), WFA histochemistry images (C,D,I,J), and merged images (E,F,K,L) are shown. Arrows in (H) indicate 5-HT nerve terminals on NeuN-positive motoneurons. Many 5-HT varicosities were also observed in (H,L). Scale bars = 50 μm.